出版社：A novel cloning system was developed to identify the gene which may have genetical functions involved in the segregational stability of plasmids at cell divison in Bacillus subtilis. By a double-crossover, a lacZ gene was in tegrated on to the chromosome of JH642 to generate two new strains JH256 and JH 257, in which the lacZ gene was completely regulated by spac OA promoter. Many plasmids with lac I or lacI gene on the genome have been constructed, but only the plasmids which are deficient in t
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